Our experience tells us, that the best results are obtained when we understand how the protein is implemented in your application. We ensure that the construct is verified by DNA sequencing.
The palette of expression systems in our facilities includes E. Coli such as Rosetta, BL21 (DE3) and SHuffle E, and several mammal cell systems: Expi293f, ExpiCHO. We
Protein purification in Ovodan Biotech facilities is based on our automated chromatographic technology using customized ÄKTA equipment. The purification strategy is carefully implemented in the vector design to ensure as few steps as possible.
As standard our QC includes, SDS page and ELISA if antibodies are available. However, we strongly recommend mass spectrometry to verify aa-sequence and identify glycosylation sites. This expertise we are also able to provide.